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mucin2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mucin2
    Mucin2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mucin2/Mucin+2+Antibody/us12584112-444-13-14
    Average 96 stars, based on 553 article reviews
    mucin2 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Arbutin alleviates intestinal colitis by regulating neutrophil extracellular traps formation and microbiota composition.
    Article Snippet: Background: Ulcerative colitis (UC) is a chronic recurrent intestinal disease lacking effective treatments. β-arbutin, a glycoside extracted from the Arctostaphylos uva-ursi leaves, that can regulate many pathological processes.. However, the effects of β-arbutin on UC remain unknown.. Purpose: In this study, we investigated the role of β-arbutin in relieving colitis and explored its potential mechanisms in a mouse model of dextran sulfate sodium (DSS)–induced colitis.

    Staining:

    Article Title: Arbutin alleviates intestinal colitis by regulating neutrophil extracellular traps formation and microbiota composition.
    Article Snippet: Background: Ulcerative colitis (UC) is a chronic recurrent intestinal disease lacking effective treatments. β-arbutin, a glycoside extracted from the Arctostaphylos uva-ursi leaves, that can regulate many pathological processes.. However, the effects of β-arbutin on UC remain unknown.. Purpose: In this study, we investigated the role of β-arbutin in relieving colitis and explored its potential mechanisms in a mouse model of dextran sulfate sodium (DSS)–induced colitis.

    other:

    Article Title: 3D fluorescence staining and confocal imaging of low amount of intestinal organoids (enteroids): Protocol accessible to all
    Article Snippet: Mucin2 , MUC2 , 1:200 , 1 μg/ml , Mouse , Santa Cruz , Sc-515032 , AB_2815005.

    Incubation:

    Article Title: Old mitochondria regulate niche renewal via α-ketoglutarate metabolism in stem cells.
    Article Snippet: .. The primary antibodies lysozyme (EC3.2.1.17, 1:500 dilution, Dako), E-cadherin (1:500 dilution, catalogue no. 610181, BD Biosciences), chromogranin A (1:500 dilution, ab15160, Abcam), Mucin2 (1:500 dilution, catalogue no. sc-15334, Santa-Cruz Biotechnology) and 5hmC (1:500 dilution, catalogue no. AB_10013602, Active Motif) were incubated over night at +4 °C and detected with Alexa Fluor 488/594/633/647-conjugated anti-rabbit, anti-rat or anti-mouse secondary antibodies (1:500 dilution, Thermo Fisher Scientific) using a 1-h incubation at room temperature. .. Nuclei were co-stained with DAPI (1 μg ml−1, Thermo Fisher Scientific) or Hoechst 33342 (1 μg ml−1, Thermo Fisher Scientific).

    Article Title: Old mitochondria regulate niche renewal via α-ketoglutarate metabolism in stem cells
    Article Snippet: .. The primary antibodies lysozyme (EC3.2.1.17, 1:500 dilution, Dako), E-cadherin (1:500 dilution, catalogue no. 610181, BD Biosciences), chromogranin A (1:500 dilution, ab15160, Abcam), Mucin2 (1:500 dilution, catalogue no. sc-15334, Santa-Cruz Biotechnology) and 5hmC (1:500 dilution, catalogue no. AB_10013602, Active Motif) were incubated over night at +4 °C and detected with Alexa Fluor 488/594/633/647-conjugated anti-rabbit, anti-rat or anti-mouse secondary antibodies (1:500 dilution, Thermo Fisher Scientific) using a 1-h incubation at room temperature. .. Nuclei were co-stained with DAPI (1 μg ml −1 , Thermo Fisher Scientific) or Hoechst 33342 (1 μg ml −1 , Thermo Fisher Scientific).

    Article Title: Methylmercury Chronic Exposure and a High-Fat Diet Induce Gut Microbiome Alterations and Intestinal Barrier Disruption in Mice.
    Article Snippet: Methylmercury (MeHg) is markedly toxic to humans.. Our study explores whether MeHg and highfat diet (HFD) can impair the intestinal barrier with microbiota dysbiosis in mice.. Weanling mice were fed to HFD or standard diet for 40 days.

    Article Title: Organoid tissue engineering
    Article Snippet: .. After rinsing with PBS, cells were incubated with primary antibodies against Lysozyme (Pierce), Mucin2 (Santa Cruz) and chromogranin A (Santa Cruz) diluted in PBS overnight, at 4° C. Cells were washed 3×1 hr with 0.05% Tween 20 in PBS followed by incubation with the appropriate Alexa-conjugated secondary antibody, Alexa-conjugated phalloidin and DAPI (Molecular Probes) diluted in PBS overnight, at 4° C. Cells were further washed 3×1 hr with 0.05% Tween 20 in PBS, with agitation, before addition of mounting media (Prolong Diamond, Molecular Probes). ..



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    APELNs protect the intestinal barrier in DSS-induced colitis. (A) AB/PAS (Alcian Blue/Periodic Acid-Schiff stain) staining of colon sections. (B) Quantification of goblet cells in colonic crypts. (C) APELNs reduced intestinal permeability. (D–G) qRT-PCR analysis of Claudin-1, ZO-1, <t>MUC2,</t> and OCLN expression in colon samples. (H, I) IF showing the localization of Claudin-1, ZO-1, MUC2, and OCLN. (J, K) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in colon tissues. Data were shown as mean ± standard deviation. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. AB/PAS: Alcian blue/periodic acid-schiff stain; APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; DSS: Dextran sodium sulfate; FITC-Dex: Fluorescein isothiocyanate-dextran permeability assay; IF: Immunofluorescence; MUC2: <t>Mucin2;</t> ns: Not significant; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; WB: Western blotting; ZO-1: Zonula occludens-1.
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    Image Search Results


    APELNs protect the intestinal barrier in DSS-induced colitis. (A) AB/PAS (Alcian Blue/Periodic Acid-Schiff stain) staining of colon sections. (B) Quantification of goblet cells in colonic crypts. (C) APELNs reduced intestinal permeability. (D–G) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN expression in colon samples. (H, I) IF showing the localization of Claudin-1, ZO-1, MUC2, and OCLN. (J, K) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in colon tissues. Data were shown as mean ± standard deviation. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. AB/PAS: Alcian blue/periodic acid-schiff stain; APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; DSS: Dextran sodium sulfate; FITC-Dex: Fluorescein isothiocyanate-dextran permeability assay; IF: Immunofluorescence; MUC2: Mucin2; ns: Not significant; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; WB: Western blotting; ZO-1: Zonula occludens-1.

    Journal: Chinese Medical Journal

    Article Title: “One stone three birds”: Andrographis paniculata -derived exosome-like nanoparticles mitigate dextran sulfate sodium-induced colitis

    doi: 10.1097/CM9.0000000000003651

    Figure Lengend Snippet: APELNs protect the intestinal barrier in DSS-induced colitis. (A) AB/PAS (Alcian Blue/Periodic Acid-Schiff stain) staining of colon sections. (B) Quantification of goblet cells in colonic crypts. (C) APELNs reduced intestinal permeability. (D–G) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN expression in colon samples. (H, I) IF showing the localization of Claudin-1, ZO-1, MUC2, and OCLN. (J, K) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in colon tissues. Data were shown as mean ± standard deviation. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. AB/PAS: Alcian blue/periodic acid-schiff stain; APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; DSS: Dextran sodium sulfate; FITC-Dex: Fluorescein isothiocyanate-dextran permeability assay; IF: Immunofluorescence; MUC2: Mucin2; ns: Not significant; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; WB: Western blotting; ZO-1: Zonula occludens-1.

    Article Snippet: Samples were incubated overnight at 4°C with primary antibodies, including anti-Mucin2 (MUC2) (GB11344, Servicebio, China), anti-occludin (OCLN) ( GB111401 , ab216327, Servicebio), anti-ZO-1 ( GB111402 , Servicebio), and anti-Claudin-1 (GB15032, Servicebio).

    Techniques: Staining, Permeability, Quantitative RT-PCR, Expressing, Standard Deviation, Derivative Assay, FITC-Dextran Permeability Assay, Immunofluorescence, Reverse Transcription, Polymerase Chain Reaction, Western Blot

    APELNs enhance TJ protein expression in IECs. (A, B) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (C, D, E) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (F, G) IF images showing the expression of Claudin-1, ZO-1, MUC2, and OCLN. Bar: 20 μm. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; IEC: Intestinal epithelial cell; LPS: Lipopolysaccharide; MUC2: Mucin2; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; TJ: Tight junction; WB: Western blotting; ZO-1: Zonula occludens-1.

    Journal: Chinese Medical Journal

    Article Title: “One stone three birds”: Andrographis paniculata -derived exosome-like nanoparticles mitigate dextran sulfate sodium-induced colitis

    doi: 10.1097/CM9.0000000000003651

    Figure Lengend Snippet: APELNs enhance TJ protein expression in IECs. (A, B) qRT-PCR analysis of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (C, D, E) WB analysis and relative protein expression of Claudin-1, ZO-1, MUC2, and OCLN in NCM460 and HCT116 cells. (F, G) IF images showing the expression of Claudin-1, ZO-1, MUC2, and OCLN. Bar: 20 μm. * P <0.05, † P <0.01, ‡ P <0.001, § P <0.0001. APELN: Andrographis paniculata -derived exosome-like nanoparticle; DAPI: 4′,6-diamidino-2-phenylindole; IEC: Intestinal epithelial cell; LPS: Lipopolysaccharide; MUC2: Mucin2; OCLN: Occludin; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; TJ: Tight junction; WB: Western blotting; ZO-1: Zonula occludens-1.

    Article Snippet: Samples were incubated overnight at 4°C with primary antibodies, including anti-Mucin2 (MUC2) (GB11344, Servicebio, China), anti-occludin (OCLN) ( GB111401 , ab216327, Servicebio), anti-ZO-1 ( GB111402 , Servicebio), and anti-Claudin-1 (GB15032, Servicebio).

    Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot